Fee-For-Service Screening

Dear Customer,

In a response to the rapidly increasing popularity of our screening services we have developed a new client focused process. The first step of this process is to make sure that from the very beginning of the project we align your screening needs with the various service packages that we offer.

This insures that we deliver on your screening objectives in a fast, reliable and focused manner. In order to achieve this, we have created this on line form to help us better understand your screening needs and objectives straight from the start.

So please take a few minutes to register and fill this form detailing your screening requirements! Once you have sent this form on line, you will receive an email confirmation of your request. A member of the Solvo Business Development team will then get in touch with you shortly to discuss the details of the project.

Thank you for using our form!

Fee-For-Service Screening Request Form

We recommend this type of service for customers who would like to test a limited number of compounds (typically <20) more thoroughly by directly utilizing Solvo Biotechnology in vitro testing experience. Typically these assays are performed on compounds in lead optimization or at a later stage, in drug development (preclinical or clinical). We are ready to handle larger compound sets too, however, for large scale screening we suggest to establish these assays in house. We have great expertise in assisting our customers in adapting these assays for in house applications.

ABC Transporter interaction testing might help to understand the mechanism behind the ambiguous ADME/Tox profile of certain drug candidates.

In our lab, we can perform all the ABC transporter measurements listed on our Membrane Preparations page:

A. ABC (efflux) Transporter Assays

1. ATPase Assays [ATPase activation + inhibition]

Substrate / inhibitor used: test drug or test drug + posCTRL

Deliverables: EC₅₀, IC₅₀

Suggested concentration range of test drug: 137 nM – 300 µM

¹ Mutant variant (not wild type)

2. Vesicular Transport Assays

Deliverables: IC₅₀ – for interacting compounds

(Follow-up studies are available, which provide kinetic data: K_m, K_i and V_max)

Concentration range of test drug: 0.41 µM – 300 µM

3. Dye Transport Assays

Deliverables: IC₅₀

Suggested concentration range of test drug: 0,07 µM – 150 µM

4. Nucleotide Trapping

Available for MDR1, MRP1, MRP2, rat Mrp2, MXR and MDR2 (MDR3). Costs are individually based.

B. Uptake Transporter Assays

C. Monolayer Assays

Monolayer systems consist of a tight cell layer grown on a porous support to separate two fluid compartments. They are widely regarded as the most sophisticated in vitro tools for medium to high throughput modelling of important pharmacokinetic barriers, such as intestinal epithelium, blood-brain barrier etc (see Hamilton RD et al. 2007. for a recent application).
Two systems that are applied widely in monolayer studies are the human colon carcinoma cell line Caco-2 and transfectant MDCKII cells. SOLVO offers several options for both systems.

Caco2 screening services:

1. Passive permeability studies
2. Specific transporter studies
3. Drug-Drug Interaction studies

SOLVO development pipelin also includes the following elements: Vectorial assays, Physiological Barrier Models, Toxicity Microarray, Transporter chip, Transporter humanized knock-out and knock-in animals. Different new assays are under development for several other human and animal ABC efflux and uptake transporters.

General Remarks

Screening results are usually available within 4 to 10 weeks. The schedule might depend on the actual workload of SOLVO’s laboratory. We can provide a final schedule before contracting, including needed quantity of test drugs. Performing inhibition and activation assays is always strongly recommended.

Assays are carried out in duplicates. Upon request of customer, assays can be carried out in triplicates. Positive controls are also available.

We provide a detailed report at the end of each task, including a guide for conclusions.