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June 2004 |
| Newsletter | ||
SOLVO
Biotechnology is happy to announce that, as a result of our recent development
efforts, we have expanded our
portfolio with three new
products: rat
Mdr1 membrane vesicles and control vesicles for studies on Sf9 and Mammalian
membrane preparations.
Studies
of compounds in parallel with both human MDR1 and rat Mdr1 membrane assays open
a new opportunity in ADMET. The rat
Mdr1b transporter is the rat homolog of the human MDR1 protein. The rat
Mdr1b vesicles offer a new, better-defined assay system to study the interaction
of pharmacological agents with the rat Mdr1b transporter. In transport studies
it transports the same substrates with similar kinetic constants (see Hooiveld et al.,
2002). Using a set of 80 compounds we successfully proved that the
rat Mdr1b and the human MDR1 have similar substrate specificity in the ATPase assay (manuscript
under preparation).
The MDR1
protein is involved in cancer drug resistance and in the transport of
hydrophobic drugs and xenobiotics in the bowel,
kidney, liver and the blood-brain barrier (see Thiebaut et al., 1987,
van Helvoort et al., 1996, Cordon-Cardo et al., 1989, Rao et al.,
1999, see Borst and Elferink, 2002).
SB-beta-gal-Sf9-CTRL membranes
are purified from Sf9 cells infected with a baculovirus construct containing the gene of the β-galactosidaze enzime. The membrane
preparation does not contain any known human or mammalian ABC transporters.
Therefore, the vesicles can be used both in the ATPase
assay and the vesicular transport assay as control vesicles.
SB-M-CTRL membranes are purified from mammalian cells that were not selected for ABC transporter expression. The membrane preparation contains three orders of magnitude less MXR (ABCG2) than SB-MXR-M-ATPase or SB-MXR-M-VT membranes, as detected by Western blot. The low protein expression does not yield any detectable function in either the ATPase assay or the vesicular transport assay. Therefore, the vesicles can be used both in the ATPase assay and the vesicular transport assay as control vesicles.
We
attached our updated Membrane Product
Catalogue and Fee-For-Service
Screening Catalogue for your review. For further
information please check our detailed Assay Protocols and Sample Datasheets at our recently updated website: www.solvo.com
We hope to hear from you at your earliest convenience.
With Kindest Regards,
The SOLVO
Transporter Assay Team:
Csengele
Barta PhD, Production Manager
Péter A. Csíkos MBA, Senior Business
Development Associate
Arnold Fehér MD, MSc, Director of Business Development
Hristos Glavinas,
Product Development Manager
Rita Kiraly, Business
Development Associate
Ildikó Kokovai, Logistics
Coordinator / Order Entry
Péter Krajcsi, PhD, Chief Scientific
Officer
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For Customers in Japan:
Funakoshi (distributor of SOLVO membrane products)
Iwaki (exclusive distributor of SOLVO Fee-for Service Screenings, distributor of SOLVO membrane products)
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