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6 September 2006 |
| Newsletter | ||
SOLVO Biotechnology is launching the new Mouse Bsep and MRP5 Vesicular transport assays now!
The newest ABC membrane transporter products - Mouse Bsep and MRP5 vesicular transport assays are now available directly from SOLVO or from our distributors.
The standard Vesicular Transport assay is an indirect inhibitory-type assay, and is performed with cold test compounds. It provides information on any interaction between the ABC transporter and the test drug that would affect the transport of the reporter substrate (radioactive compound). In this assay the transport of a known substrate - the reporter substrate - is measured in the presence of the test drug. Values are presented on a relative scale with 100% defined as transport in the absence of the test compound (no inhibition), and 0% defined as transport measured in the absence of ATP (no transporter activity). IC50 is defined as the concentration required to inhibit the transport of the reporter substrate by 50%.
Mouse Bsep Vesicular Transport Assay
Taurocholate, the reporter substrate used by SOLVO's mouse Bsep VT assay, is the major endogenous substrate of Bsep. In SOLVO's indirect mouse Bsep vesicular transport assay the interaction of compounds with mouse Bsep is detected as the inhibition of the transport of 3H-Taurocholate, therefore the assay shows the effect of the test drug on the physiological function of the transporter. The assay utilizes Sf9 cell membranes containing mouse Bsep. Expession of the protein is achieved by baculoviral transfection.
MRP5 Vesicular Transport Assay
The endogenous substrates of MRP5 are cyclic nucleoside monophosphates, such as cAMP and cGMP.
Therefore, MRP5 may play a role in the regulation of signal transduction by
transporting these second messengers out of the cells. As MRP5 is present in
blood vessels, inhibition of cGMP transport can elongate the duration of
vasodilatation, making MRP5 a target in conditions like hypertonia, angina
pectoris, congestive heart failure, and acute pulmonary edema and erectile
dysfunction.
SOLVO's protocol for measuring vesicular transport on mammalian cell membranes expressing MRP5
utilizes 3H-cGMP as
substrate.
The mouse Bsep and MRP5 Vesicular Transport Assays are either available as a products (SB-mouseBsep-Sf9-VT and SB-MRP5-HEL293-VT membrane preparation) for in house screening or as the Fee-For-Service Screening performed in the SOLVO Screening Laboratory.
Best regards,
SOLVO Biotechnology
Business Development Team
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For Customers in Japan:
Funakoshi (distributor of SOLVO membrane products)
Iwaki (exclusive distributor of SOLVO Fee-for Service Screenings, distributor of SOLVO membrane products)
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