New products from SOLVO

SOLVO Biotechnology introduces

ratMrp2 PREDIVEZ^™ Vesicular Transport kit
PrediScreen^™ rat in vivo liver studies

New ratMrp2 PREDIVEZ^™ Vesicular Transport Kit

The ratMrp2 assay kit operates with fluorescent 5(6)-Carboxy-2',7'-dichlorofluorescein (CDCF) probe (reporter) substrate. A representation of the assay is shown in Figure 1.

Figure 1. Effect of drugs on rat Mrp2 mediated CDCF transport measured in the vesicular transport assay.

The PREDIVEZ^™ Vesicular Transport kit is a simple and powerful tool to investigate drug transporter interactions. It provides information on any interaction between the ABC transporter and the drug candidate that would affect the transport of the fluorescent or radiolabeled probe substrate.

PREDIVEZ^™ vesicular transport kits are available in three sizes 3, 6 and 9 compound sizes. Kits contain sufficient amount of reagents and membrane preparation for testing 3, compounds per 96-well plate. Each kit is accompanied by a CD that includes the assay protocol, Excel spreadsheet with data processing template, MSDS and inhibition curves with reference compounds.

Rat in vivo liver studies including functional testing of ratMrp2, ratBsep and ratBcrp transporters

In vivo biliary efflux experiments are performed on male Wistar rats kept under standard laboratory conditions. All procedures are approved by the Institutional Animal Care and Use Committee. Tracer doses of 3H-Estradiol-17-b-D-glucuronide, 3H-Taurocholate, 3H-Estrone-3-sulfate, probe substrates for Mrp2, Bsep and Bcrp, respectively are coadministered with the test compounds i.p. Control rats receive the probe substrates and the vehicle only. Bile samples are collected, weighted and analyzed by liquid scintillation. Interactions with the transport proteins are determined from the altered biliary elimination of the probe substrates. A representation of the assay is shown in Figure 2.

Figure 2. Inhibition of taurocholate transport of ratBsep transporters by cyclosporin A in in vivo experiments

Should you have any further questions, please contact us at sales@solvo.com.

With our best regards,
SOLVO Biotechnology
Business Development Team

CONTACT INFORMATION:

SOLVO Biotechnology
2 Gyár St. H-2040 Budaörs, HUNGARY
E-mail: sales@solvo.com
web: www.solvo.com
Phone: +36-23/503-940
Fax: +36-23/503-941

SOLVO distributors:
in US and Canada: XenoTech LLC www.xenotechllc.com,
in Europe: SOLVO and Tebu-bio www.tebu-bio.com,
in Japan: Nosan www.nosan.co.jp,
in India: Krishgen www.krishgen.com

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