Dec 2005
	Newsletter

SOLVO Biotechnology is launching new Control Membrane Products!

Dear SOLVO Customer:
We are happy to announce that SOLVO Biotechnology Inc. is launching new Control Membrane Products!

Control membranes are used to determine whether the measured activity is a real activity of the transporter. SOLVO offers control membranes for experiments carried out with sf9 and with mammalian vesicles.

Sf9 Control Vesicles

The defMRP and defMXR membranes are purified from Sf9 cells containing MRP or MXR mutant transporter which is not able to bind  the substrates (neither the activators nor the inhibitors) of the transporter. The def MXR membrane is a specific control membrane for MXR (BCRP/ABCG2) Sf9 assays. The defMRP membrane can be used as a control membrane for all experiments carried out with the other Sf9 membrane vesicles.

SB-beta-gal-Sf9-CTRL membranes are purified from Sf9 cells infected with a recombinant baculovirus  containing the gene of the beta-galactosidaze enzime. The membrane preparation does not contain any known human or mammalian ABC transporters. Therefore, the vesicles can be used both in the ATPase assay and the vesicular transport assay as control vesicles.

ATPase assay
These membrane vesicles contain a low (2-5 nmol Pi/mg membrane protein/min) vanadate sensitive ATPase activity that is attributed to vanadate sensitive ATPases different from ABC transporters. This baseline activity cannot be further activated by compounds that interact with ABC transporters. When setting up the control experiment use the same protocol (e.g. amount of membrane protein, incubation time, etc.) as for the vesicles that contain the transporter of your interest.

Vesicular transport assay
The membrane vesicles show no ATP dependent uptake of the compounds used for studies with vesicles containing ABC transporters. When setting up the control experiment use the same protocol (e.g. amount of membrane protein, incubation time, etc.) as for the vesicles that contain the transporter of your interest.

Mammalian Control Vesicles

ATPase assay
These membrane vesicles do not show any vanadate sensitive ATPase activity with and without the presence of compounds that interact with ABC transporters. When setting up the control experiment use the same protocol (e.g. amount of membrane protein, incubation time, etc.) as for the vesicles that contain the transporter of your interest.

Vesicular transport assay
The membrane vesicles show no ATP dependent uptake of the compounds used for studies with vesicles containing ABC transporters. When setting up the control experiment use the same protocol (e.g. amount of membrane protein, incubation time, etc.) as for the vesicles that contain the transporter of your interest.

Available control membrane products for studies on Sf9 membranes:

Available control membrane products for studies on mammalian membranes:

If you have any questions about our products and services, please do not hesitate to contact us!

Best regards,
SOLVO Biotechnology
Business Development Team

For Customers in Japan: Funakoshi (distributor of SOLVO membrane products) Iwaki (exclusive distributor of SOLVO Fee-for Service Screenings, distributor of SOLVO membrane products)

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