Solvo's core competence: providing tools for Efflux and Uptake Transporter Assays in ADMETox Research
Speeding up drug discovery and development
Applications of transporter assays
How to benefit from our assays?
Solvo: the transporter company
Partnering with Solvo
Cooperation / service business models on drug transporters

Solvo's core competence: providing tools for Efflux and Uptake Transporter Assays in ADMETox Research

Figure 1: The cell membrane
(Source: http://www.biosci.uga.edu/almanac/bio_103/notes/may_15.html)

SOLVO offers drug discovery products and services to pharmaceutical companies, relevant to many therapeutic areas. Our in vitro efflux transporter assays and cell lines expressing various uptake transporter proteins answer important questions regarding the interactions between drug compounds and a wide array of transporters.
Moreover, SOLVO Biotechnology offers membrane vesicles prepared from Sf9 (Spodoptera frugiperda ovarian) cells or mammalian cell lines.

The membrane vesicles contain a wide range of different transporters and can be utilized in various assays. SOLVO Biotechnology provides four different solutions for ABC (xenobiotic) and uptake transporter assays to be used in R&D:

Membrane Preparations - for ATPase Assay and Vesicular Transport Assay (direct uptake) measurements Fee-For-Service Screening Sublicense for the Calcein Assay Custom-Made Expression and Screening Systems

Speeding up drug discovery and development

These solutions can significantly speed up the drug discovery and development process. Efflux and uptake transporter assay results - together with physicochemical data and/or monolayer efflux test results - provide an ADME prediction at relatively low cost before the compounds would enter expensive preclinical animal testing. Therefore, application of this technology is a step towards the "fail fast, fail cheap" drug discovery and development philosophy. Another added advantage of our assays over animal testing is that it allows for monitoring interaction of drugs and drug candidates with human proteins.

Applications of transporter assays

Figure 2: The human cells
(Source: http://www.biocenter.hu)

The three major applications of ABC Transporter assays are:

Predicting the penetration of test compounds through the pharmacological barriers (blood-brain barrier, intestinal mucosa, kidney, liver etc.) = in vitro predictive ADME, Predicting the penetration of intracellularly active cytostatics into the cancer cells, Testing of functional effect of MDR modulators.

How to benefit from our assays?

SOLVO's mainstream products can be used for:

Expression of a wide array of human ABC transporters (MDR1, MDR2/3, MRP1, MRP2, MRP3, MRP4, MRP5, MRP6, MXR, BSEP and others) and rodent (rat, mouse) transporters in various expression systems (e.g. baculovirus-based insect cell) Functional characterization of proteins (ATPase, transport and binding data) Identification of new drug targets on ABC transporters. Development of various cell lines expressing a range of pharmacologically relevant uptake transporter proteins

Our unique technology platform is characterized by the following attributes:

Flexible collaboration models for Client focused Services and Reagent sales Cost-efficiency Reliability, as only one transporter is expressed in each individual reagent at one time Easy-to-use methodology with detailed HT protocols available for all reagents (including all insect and mammalian membrane assays)

A wide array of ABC transporter assays and cell lines expressing various uptake transporter proteins assays are available from SOLVO. SOLVO is constantly developing new assays, for additional ABC and for other transporters as well.
The patented Calcein Assay, ATPase Assay and some Vesicular transport assays are optimized for high-throughput screening on 96 well plates and widely used by pharmaceutical companies. SOLVO provides full technology transfer for in-house use as well as fee-for-service screening.

Solvo: the transporter company

Partnering with Solvo

Partnering is a key part of our research and business strategy. Our group is committed to collaborating with others, developing long-term partnerships that leverage each company's strengths, and bringing significant advances to researchers worldwide.

SOLVO is interested in collaborations, which may take many forms:

Products- co-development, product acquisitions and/or co-promotion/co-marketing arrangements  Strategic partnerships that build on complementary capabilities to develop comprehensive Technology Platforms Broad based research collaborations, including alternative discovery initiatives and new collaborative model In-licensing compounds, technologies or other intellectual property from Academic and Industrial Research laboratories and out-licensing our technology from the R&D pipeline Academic liaisons

Cooperation / service business models on drug transporters