Membrane preparations
SOLVO Biotechnology offers membrane preparations produced from Sf9 (Spodoptera frugiperda ovarian) cells or mammalian cell lines expressing high levels of a selected human ABC transporter protein. Control membranes with no, or significantly lower transporter activity are also available.
Our products are offered either as easy-to-use kits or membrane preparations to support in vitro studies to ascertain whether a drug candidate is a substrate for, or inhibitor of a specific transporter.
Each product goes with individual signed data sheet, detailed assay protocol and technical support via e-mail or phone.
Membranes are provided in 2,5 mg (0,5 ml) units and are stored at -80°C and shipped on dry ice.
Vesicular transport membranes
Control membranes
Membrane preparations for ATPase assays
| Catalogue # | Transporter (gene) |
Intended use | Probe (reference substrate) | Membrane1 use per assay well | Basal ATPase activity2 [nmol Pi/mg/min] | Modified ATPase activity2,3 [nmol Pi/mg/min] | -fold activation |
| SB-MDR1-Sf9-ATPase | MDR1/PgP (ABCB1) |
ATPase activation and inhibition | Verapamil (40 μM) | 40 μg | 8-22 | 30-90 | 3-5 |
| SB-ratMdr1b-Sf9-ATPase | rat Mdr1b | ATPase activation and inhibition | Verapamil (200 μM) | 20 μg | 10-25 | 70-100 | 4-8 |
| SB-MRP1-Sf9-ATPase | MRP1 (ABCC1) |
ATPase activation and inhibition | NEM-GS (10 mM) | 40 μg | 5-10 | 10-25 | 2-3 |
| SB-MRP2-Sf9-ATPase | MRP2 (ABCC2) |
ATPase activation and inhibition | Probenecid (1 mM) | 20 μg | 5-15 | 20-40 | 2-4 |
| SB-MRP3-Sf9-ATPase | MRP3 (ABCC3) |
ATPase activation and inhibition | Benzbromarone (50 µM) | 20 μg | 6-13 | 18-30 | 2-4 |
| SB-BCRP-M-ATPase | BCRP / MXR (ABCG2) | ATPase activation | Sulfasalazine (10 µM) | 20 μg | 5-10 | 15-40 | 2-4 |
| ATPase inhibition | Hoechst 33342 (10 µM) / Ko134 (100 nM) | 0-3 | 0-1 | ||||
| SB-BCRP-R482G-Sf9-ATPase | BCRP / MXR-R482G (ABCG2-R482G)4 |
ATPase activation | Prazosine (50 µM) | 20 μg | 30-80 | 50-150 | 1.5-2 |
| ATPase inhibition | Hoechst 33342 (10 µM) / Ko134 (100 nM) | 5-25 | 0-1 |
2. Vanadate-sensitive
3. In the presence of the reference substrate
4. Mutant version (R482G)
For scientific background of ATPase assays please click here.
Membrane preparations for Vesicular Transport assays
| Catalogue # | Transporter (gene) | Probe (reference substrate) | Membrane1 use per assay well | ATP dependent transport [pmol /mg/min] |
| SB-MDR1-K-VT | MDR1/P-gp (ABCB1) | 3H-NMQ | 50 μg | 350-500 |
| SB-ratMdr1b-Sf9-VT | ratMdr1b | 3H-NMQ | 50 μg | 30-80 |
| SB-MRP1-Sf9-VT | MRP1 (ABCC1) | 3H-LTC4 (50 nM) | 50 μg | 20-45 |
| SB-MRP2-Sf9-VT | MRP2 (ABCC2) | 3H-E217βG (50 µM) | 50 μg | 500-1000 |
| 3H-LTC4 (50 nM) | 6-20 | |||
| SB-ratMrp2-HEK293-VT | rat Mrp2 | 3H-E217βG (1 µM & 100 µM) | 50 μg | 7-20 (1 µM 3H-E217βG) & 600-1000 (100 µM 3H-E217βG) |
| SB-MRP3-Sf9-VT | MRP3 (ABCC3) | 3H-E217βG (1 µM) | 50 μg | 20-35 |
| SB-MRP4-LLC-PK1-VT | MRP4 (ABCC4) | 3H-DHEAS (0,02 µM) | 50 μg | |
| SB-MRP5-HEK293-VT | MRP5 (ABCC5) | 3H-cGMP (1 µM) | 30 µg | 0.6-1.2 |
| SB-BCRP-Sf9-VT | BCRP / MXR (ABCG2) | 3H-Methotrexate (100 µM) | 50 μg | 35-65 |
| SB-BCRP-M-VT | BCRP / MXR (ABCG2) | 3H-Estrone-3-sulfate (1 µM) | 25 μg | 40-100 |
| 3H-Methotrexate (100 µM) | 50 μg | 40-90 | ||
| SB-BSEP-Sf9-VT | BSEP (ABCB11) | 3H-Taurocholate (2 µM) | 50 μg | 25-45 |
| SB-mouseBsep-Sf9-VT | mouse BSEP | 3H-Taurocholate (2 µM) | 50 μg | 100-400 |
For scientific background of vesicular transport assays please click here.
Control membranes
| Catalogue # | Transporter | Intended use |
| SB-defMRP-Sf9-CTRL | Defective MRP | Control for MRP1, MRP2, ratMrp2, MRP3 assays |
| SB-defBCRP-HAM-Sf9-CTRL | Defective BCRP | Control for BCRP assays |
| SB-M-CTRL | lack of BCRP | Control for BCRP-M assays |
| SB-Beta-gal-Sf9-CTRL | no transporter | Control for all efflux transporter assays |
| SB-LLC-PK1-CTRL | lack of MRP4 | Control for MRP4 assay |
| SB-HEK293-CTRL | lack of MRP5 or rat Mrp2 | Control for MRP5 and rat Mrp2-HEK293-VT assays |
| SB-defPgp-Sf9-CTRL | Defective MDR1 | Control for MDR1-Sf9 and ratMdr1b-Sf9 assays |
| SB-K-CTRL | lack of MDR1 | Control for MDR1-K assays |
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Tel.: +36 23 503 940
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