Membrane preparations

SOLVO Biotechnology offers membrane preparations produced from Sf9 (Spodoptera frugiperda ovarian) cells or mammalian cell lines expressing high levels of a selected human ABC transporter protein. Control membranes with no, or significantly lower transporter activity are also available.

Our products are offered either as easy-to-use kits or membrane preparations to support in vitro studies to ascertain whether a drug candidate is a substrate for, or inhibitor of a specific transporter.

Each product goes with individual signed data sheet, detailed assay protocol and technical support via e-mail or phone.

Membranes are provided in 2,5 mg (0,5 ml) units and are stored at -80°C and shipped on dry ice.

ATPase membranes
Vesicular transport membranes
Control membranes



Membrane preparations for ATPase assays

Catalogue # Transporter
(gene)
Intended use Probe (reference substrate) Membrane1 use per assay well Basal ATPase activity2 [nmol Pi/mg/min] Modified ATPase activity2,3 [nmol Pi/mg/min] -fold activation
SB-MDR1-Sf9-ATPase MDR1/PgP
(ABCB1)
ATPase activation and inhibition Verapamil (40 μM) 40 μg 8-22 30-90 3-5
SB-ratMdr1b-Sf9-ATPase rat Mdr1b ATPase activation and inhibition Verapamil (200 μM) 20 μg 10-25 70-100 4-8
SB-MRP1-Sf9-ATPase MRP1
(ABCC1)
ATPase activation and inhibition NEM-GS (10 mM) 40 μg 5-10 10-25 2-3
SB-MRP2-Sf9-ATPase MRP2
(ABCC2)
ATPase activation and inhibition Probenecid (1 mM) 20 μg 5-15 20-40 2-4
SB-MRP3-Sf9-ATPase MRP3
(ABCC3)
ATPase activation and inhibition Benzbromarone (50 µM) 20 μg 6-13 18-30 2-4
SB-BCRP-M-ATPase BCRP / MXR (ABCG2) ATPase activation Sulfasalazine (10 µM) 20 μg 5-10 15-40 2-4
ATPase inhibition Hoechst 33342 (10 µM) / Ko134 (100 nM) 0-3 0-1
SB-BCRP-R482G-Sf9-ATPase     BCRP / MXR-R482G
(ABCG2-R482G)4
ATPase activation Prazosine (50 µM) 20 μg 30-80 50-150 1.5-2
ATPase inhibition Hoechst 33342 (10 µM) / Ko134 (100 nM) 5-25 0-1
1. Recommended values
2. Vanadate-sensitive
3. In the presence of the reference substrate
4. Mutant version (R482G)

For scientific background of ATPase assays please click here.


Membrane preparations for Vesicular Transport assays

Catalogue # Transporter (gene) Probe (reference substrate) Membrane1 use per assay well ATP dependent transport [pmol /mg/min]
SB-MDR1-K-VT MDR1/P-gp (ABCB1) 3H-NMQ 50 μg 350-500
SB-ratMdr1b-Sf9-VT ratMdr1b 3H-NMQ 50 μg 30-80
SB-MRP1-Sf9-VT MRP1 (ABCC1) 3H-LTC4 (50 nM) 50 μg 20-45
SB-MRP2-Sf9-VT MRP2 (ABCC2) 3H-E217βG (50 µM) 50 μg 500-1000
3H-LTC4 (50 nM) 6-20
SB-ratMrp2-HEK293-VT rat Mrp2 3H-E217βG (1 µM & 100 µM) 50 μg 7-20 (1 µM 3H-E217βG) & 600-1000 (100 µM 3H-E217βG)
SB-MRP3-Sf9-VT MRP3 (ABCC3) 3H-E217βG (1 µM) 50 μg 20-35
SB-MRP4-LLC-PK1-VT MRP4 (ABCC4) 3H-DHEAS (0,02 µM) 50 μg  
SB-MRP5-HEK293-VT MRP5 (ABCC5) 3H-cGMP (1 µM) 30 µg 0.6-1.2
SB-BCRP-Sf9-VT BCRP / MXR (ABCG2) 3H-Methotrexate (100 µM) 50 μg 35-65
SB-BCRP-M-VT BCRP / MXR (ABCG2) 3H-Estrone-3-sulfate (1 µM) 25 μg 40-100
3H-Methotrexate (100 µM) 50 μg 40-90
SB-BSEP-Sf9-VT BSEP (ABCB11) 3H-Taurocholate (2 µM) 50 μg 25-45
SB-mouseBsep-Sf9-VT mouse BSEP 3H-Taurocholate (2 µM) 50 μg 100-400


For scientific background of vesicular transport assays please click here.

Control membranes

Catalogue #TransporterIntended use
SB-defMRP-Sf9-CTRLDefective MRPControl for MRP1, MRP2, ratMrp2, MRP3 assays
SB-defBCRP-HAM-Sf9-CTRLDefective BCRPControl for BCRP assays
SB-M-CTRLlack of BCRPControl for BCRP-M assays
SB-Beta-gal-Sf9-CTRLno transporterControl for all efflux transporter assays
SB-LLC-PK1-CTRLlack of MRP4Control for MRP4 assay
SB-HEK293-CTRLlack of MRP5 or rat Mrp2Control for MRP5 and rat Mrp2-HEK293-VT assays
SB-defPgp-Sf9-CTRLDefective MDR1Control for MDR1-Sf9 and ratMdr1b-Sf9 assays
SB-K-CTRLlack of MDR1Control for MDR1-K assays

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Order E-mail: sales@solvo.com
Tel.: +36 23 503 940
If ordering in North America, Japan or India, please see our Distributor information
This page contains information about following topics: membrane preparations,transporter interaction,ABC transporter,Sf9 cells,substrate,inhibitor,ATPase assays,Vesicular Transport assays,Control membrane,MDR1 ATPase,ratMdr1b ATPase,MRP1 ATPase,MRP2 ATPase,ratMrp2 ATPase,MRP3 ATPase,BCRP-ATPase,MDR1 VT,ratMdr1b VT,MRP1 VT,MRP2 VT,MRP3 VT,MRP5 VT,BCRP VT,BSEP VT,mouseBsep VT,ABCB1 assay,ABCB11 assay,ABCC1 assay,ABCC2 assay,ABCC3 assay,ABCC5 assay,ABCG2 assay,Transporter Oral bioavailability, BBB penetration, Transporter CNS, Intestinal Drug Absorption, Transporter Pharmacokinetics, Transporter Pharmacodynamics.