PREDIVEZ™ Fluorescent Vesicular Transport Kit
The new PREDIVEZ™ Vesicular Transport Kit is a unique product for measuring the drug-transporter interaction in a convenient, "ready to assay" package. The PREDIVEZ™ kit operates with fluorescent reporter substrate, and contains all you need to conduct vesicular transport assays, including all necessary reagents and solutions.
SOLVO's PREDIVEZ™ Kit is available in 3-, 6- and 9-compound sizes.
The PREDIVEZ™ Vesicular Transport kit is an inhibitory assay. It provides information on any interaction between the ABC transporter and the test drug that would affect the transport of the fluorescent reporter substrate. The Fluorescent PREDIVEZ™ Kit delivers major advantages over the conventional radioactive Vesicular Transport assay | 
Representation of the vesicular transport assay |
Advantages |
The reporter substrate provides low non-specific binding, so you can get a larger signal and smaller standard deviations for your test compounds.
- Sensitive and quick fluorescent measurement
With the use of a fluorescent reporter substrate you can avoid radioactive materials and lengthy scintillation counting.
- Larger dynamic range / signal-to-background ratio compared to the radioactive Vesicular transport assay
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- Instant results
Data can be presented in transport values (with the help of the calibration curve, included), or in percentages. Negative control vesicles are also included in the kit for performance monitoring.
3 compounds in 7 concentrations can be screened using one 3-compound Kit.
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Reproducible results: - Low inter-assay variability
- Z' factor for MRP2 is ~ 0.74
- Increased automated system compatibility
Recommended equipment: - 96-well filter plates from Millipore (Cat No. MSFB6B) or equivalent
- Filtration manifold from Millipore (Cat No. MSVMHTS00) or equivalent
- Fluorescent plate reader
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Easy to use
All reagents to perform the assay are included!
Every kit comes with a CD containing important information about the
PREDIVEZ assay:
- Assay protocol
- Excel data evaluation sheet
- MSDSs
- Reference curves with known interactors
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PREDIVEZ Kits are now available for the following transporters
- MRP1 PREDIVEZ™ Kit
- MRP2 PREDIVEZ™ Kit
- rat Mrp2 PREDIVEZ™ Kit
- MRP3 PREDIVEZ™ Kit
- MRP5 PREDIVEZ™ Kit
- BCRP-M- PREDIVEZ™ Kit
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Coming up:
- BSEP PREDIVEZ™ Kit (later in 2009)
- mouseBsep PREDIVEZ™ Kit (later in 2009)
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