Caco-2 Monolayer assays
Monolayers based on Caco-2 cells (a human colon carcinoma cell line) express a wide range of transporter proteins on its cell membranes similar to those of intestinal endothelium cells (Siissalo S et al. 2007, Calcagno AM et al. 2006), thus this cell line is ideal for intestinal absorption simulations. In fact, in the last decade, the utilization of Caco-2 cells has become an industry standard for the investigation of intestinal absorption, permeability and drug-drug interactions (DDIs) (Oh DM et al. 2002.).
SOLVO Biotechnology offers Caco-2 studies as part of its PrediScreen™ service solutions. Three types of studies are available:
Caco-2 studies can be performed in different setups. SOLVO offers a number of standard setups, which differ in the amount of information derived and cost. These standard setups serve as starting points to define the optimal final study parameters.
The following standard setups are available:
| 1. Basic | - Studies at one concentration |
| 2. Extended | - Studies at several concentrations, more controls |
| 3. FDA Guidelines | - Studies according to FDA draft guidance (2006) |
These packages can be customized to suite your needs. The following table summarizes some adjustable parameters.
| Parameter | Option | Effect on price |
| Unidirectional | A-B or B-A | depends on nr of wells |
| Nr of parallels | Duplicates or more | depends on nr of wells |
| TA concentration | no limitations | no effect on price |
| Nr of TA concentrations | no limitations | depends on nr of wells |
| Sink conditions | + 1% BSA in receptor compartment | no effect on price |
| Nr of samples taken | Time points and compartments | no effect on price (if nr of samples <150) |
| Specific inhibitors | More or other inhibitors per transporter | depends on nr of wells |
For more details on the Caco2 Monolayer assays please click here
* IC50 determination for inhibition of digoxin transport is also available.