Calcein assay®, Hoechst assay (Dye transport assays)
Calcein assay® and the Hoechst assay are indirect inhibitory whole-cell assays that provide information on any interaction between the ABC transporter (MDR1/P-gp (ABCB1) or MRP1 (ABCC1) and BCRP (ABCG2)) and the test compound by measuring the change in the transport of calcein AM or Hoechst dye.
For more details on the scientific background of Calcein assays please click here.
List of transporters for dye transport assay screens:| Transporter | Cell line used | Dye used | Positive control |
| MDR1/P-gp (ABCB1) | HL60-MDR or K562-MDR | Calcein AM | Verapamil |
| MRP1 (ABCC1) | HL60-MRP | Calcein AM | MK571 |
| BCRP / MXR (ABCG2) | MXR-M | Hoechst 33342 | Proprietary |