MDCKII Monolayer assays

The introduction of transporter transfected cell lines capable of forming tight cell layers brought the possibility of investigating single transporter interactions on monolayers. MDCKII (Madin-Darby canine kidney strain II cells) cell lines have been widely used as hosts for single and/or double transfections. The difference between efflux ratios on the transfected and parental cell lines is regarded as a sign of transporter mediated active uptake or efflux process.

Single transfectant cell lines

SOLVO's single transfectant MDCKII cell lines are excellent tools to investigate specific transporter interactions for medium to high permeability test articles. These PrediScreen^™ service assays are offered in two modes, direct and indirect setups (effect of the test articles on the transport of the reporter substrate is measured).

Double transfectant cell lines

Double transfected cell lines are able to mimic physiological active vectorial transport processes such as transport of digestive products across the intestinal epithelium from the luminal to the blood side, transport of bile salts and other steroid derivatives from the blood to the biliary side in hepatocytes. PrediScreen^™ double transfected cell line based monolayer studies include the corresponding two single transfectants and the parental cell lines as controls. Double transfected monolayers are suitable for performing both direct, vectorial transport studies as well as indirect, inhibitory studies (effect of the test articles on the transport of the reporter substrate is measured).

For more details on the MDCKII Monolayer assays please click here.