High throughput assays

ATPase assay is an ideal, cost effective first assay to identify interaction between test compounds and ABC transporters. Activation and inhibition modes in combination is optimal to detect any interaction, and may identify the substrate nature of the test compound.
ATPase assays are available either as products or contract research sevices.
For scientific background of ATPase assays please click here.
For a complete list of membranes available for ATPase assays please click here.

Vesicular transport assay: "Inside-out" membrane vesicles containing ABC transporters are utilized in the vesicular transport assay to determine the IC₅₀ of the compound on the transport of a probe substrate.
The standard vesicular transport assay is an indirect inhibitory assay, performed with cold test compounds. The transport of the fluorescent or radiolabeled reporter substrate is measured in the presence of the test article. Vesicular transport assays are also offered in a direct format where the direct interaction of the test compound with the given transporter can be investigated. The vesicular transport assays are available as products or contract research sevices.
For scientific background of vesicular transport assays please click here.
For a complete list of membranes available for vesicular transport assays please click here.

Calcein Assay^® is a proprietary indirect inhibitory whole-cell assay that provides information on any interaction between the ABC transporter (MDR1/P-gp (ABCB1) or MRP1 (ABCC1)) and the test article by measuring the change in the transport of calcein AM.
For scientific background of Calcein Assay please click here.
For a complete list of dye transport assays please click here.

Uptake transporter assays are indirect inhibitory whole-cell assays performed on cell lines expressing the specific uptake transporter. Standard high throughput uptake transporter assays use cold test compounds and measure the inhibitory potential of the test drug on the transport of a known, radiolabeled or fluorescent substrate. Uptake transport assays are also offered in a direct format where the uptake of the test compound can be investigated. Uptake transporter assays are available as contract research services.
For scientific background of uptake transporter assays please click here.
For a complete list of uptake transporter assayss please click here.