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Products & Services > Products > Efflux Transporter Kits > PREDEASY™ ATPase Assay Kits & Controls

PREDEASY™ ATPase Assay Kits & Controls

Product	Transporter	Technology	Probe Substrate	Reference Inhibitor	Barrier
SB BCRP HAM Sf9 PREDEASY™ ATPase Kit	BCRP	ATPase Assay			Blood-brain-barrier Intestinal Barrier Hepatic Barrier
SB Bsep - Mouse HAM Sf9 PREDEASY™ ATPase Kit	Bsep - mouse	ATPase Assay			Hepatic Barrier
SB MDR1/P-gp PREDEASY™ ATPase Kit	MDR1/P-gp	ATPase Assay			Blood-brain-barrier Intestinal Barrier Renal Barrier Hepatic Barrier
SB Mdr1b - Rat PREDEASY™ ATPase Kit	Mdr1b - rat	ATPase Assay			Blood-brain-barrier Intestinal Barrier Renal Barrier Hepatic Barrier
SB MRP1 PREDEASY™ ATPase Kit	MRP1	ATPase Assay
SB MRP2 PREDEASY™ ATPase Kit	MRP2	ATPase Assay			Intestinal Barrier Renal Barrier Hepatic Barrier
SB MRP3 PREDEASY™ ATPase Kit	MRP3	ATPase Assay			Hepatic Barrier Intestinal Barrier
SB beta gal PREDEASY™ CTRL Kit		ATPase Assay
SB defBCRP HAM Sf9 PREDEASY™ Ctrl Kit	BCRP	ATPase Assay			Blood-brain-barrier Intestinal Barrier Hepatic Barrier
SB defMRP PREDEASY™ CTRL Kit		ATPase Assay
SB defPgp PREDEASY™ CTRL Kit	MDR1/P-gp	ATPase Assay

Description

PREDEASY^TM ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, “ready-to-assay” package.

The PREDEASY^TM kit delivers major technical improvements over the conventional ATPase assay, such as:

• Higher sensitivity
• Shorter incubations
• Excellent for HTS: higher Z-factor ~0.8
• Ready to use
• Lower preparative workload: no weighing powders, no pH adjustments
• Easier automation
• Lower cost for each data point generated

PREDEASY^TM Kits contain all reagents and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, MSDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.

PREDEASY^TM ATPase Assay Kits are available for 1, 3 and 10 plates for the transporters recommended by the FDA and the EMA.

Custom size kits are available upon request. For detailed information please contact us.

Reference:

1. Selective inhibition of MDR1 (ABCB1) by HM30181 increases oral bioavailability and 3 therapeutic efficacy of paclitaxel
   Kwak JO, Lee SH, Lee GS, Kim MS, Ahn YG, Lee JH, Kim SW, Kim KH, Lee MG