LLC-PK1 Monolayer Assays
| Service | Cell Line | Transporter | Technology | Membrane Type | Probe Substrate | Read Out | Reference Inhibitor | Positive Control | Dynamic Range |
|---|---|---|---|---|---|---|---|---|---|
| LLC-PK1-BCRP - Prazosin | BCRP |
Monolayer Assay |
Prazosin | Ko143 | |||||
| LLC-PK1-MDR1 - Digoxin | MDR1/P-gp |
Monolayer Assay |
Digoxin | Valspodar | |||||
| LLC-PK1-mMdr1a - Digoxin | Mdr1a - mouse |
Monolayer Assay |
Digoxin | Valspodar | |||||
| LLC-PK1-rBcrp - Prazosin | Bcrp - rat |
Monolayer Assay |
Prazosin | Ko143 | |||||
| LLC-PK1-rMdr1a - Digoxin | Mdr1a - rat |
Monolayer Assay |
Digoxin | Valspodar |
Description
The introduction of transporter transfected cell lines capable of forming tight cell layers has facilitated the investigation of single transporter interactions on monolayers. Epithelial-like pig kidney cell line (LLC-PK1) forms a tight monolayer on suitable transwell inserts and separates the two fluid compartments above and below it. Transporters present in the cell membrane can have crucial influence on material traffic between the two compartments. The difference between efflux ratios of the transfected and parental cell lines is regarded as a sign of transporter mediated active uptake or efflux process.
SOLVO’s single transfectant LLC-PK1 cell lines (BCRP) are excellent tools to investigate specific transporter interactions for medium to high permeability test articles. These service assays are offered in two modes, inhibitor and substrate assessment. (In the inhibitor assessment experiments the effect of the test articles on the transport of the reporter substrate is measured).
Studies available based on FDA and EMA Guidelines for Drug Interaction Studies and SOLVO’s recommendations:.
-
BCRP substrate assessment
- BCRP inhibitor assessment
LLC-PK1 monolayer studies can be performed in different setups. SOLVO offers a number of standard setups, which differ in terms of the derivable information and cost. These standard setups serve as starting points to define the optimal final study parameters. All setups contain a standard number of controls (e.g. low permeability, high permeability, transporter function).
Study setups can be customized to suit your needs.
Learn more about the background of Monolayer technologies
