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Catalog > Services > Efflux Transporter Services > Vesicular Transport Assays

Vesicular Transport Assays

Service	Transporter	Technology	Probe Substrate	Reference Inhibitor	Barrier
BCRP Vesicular Transport Assay	BCRP	Vesicular Transport Assay			Blood-brain-barrier Intestinal Barrier Hepatic Barrier
Bcrp1 - Mouse Vesicular Transport Assay	Bcrp - mouse	Vesicular Transport Assay			Blood-brain-barrier Intestinal Barrier Hepatic Barrier
BSEP Vesicular Transport Assay	BSEP	Vesicular Transport Assay			Hepatic Barrier
MDR1/P-gp Vesicular Transport Assay	MDR1/P-gp	Vesicular Transport Assay			Blood-brain-barrier Intestinal Barrier Hepatic Barrier Renal Barrier
Mdr1b - Rat Vesicular Transport Assay	Mdr1b - rat	Vesicular Transport Assay			Blood-brain-barrier Intestinal Barrier Renal Barrier Hepatic Barrier
MRP1 Vesicular Transport Assay	MRP1	Vesicular Transport Assay
MRP2 Vesicular Transport Assay	MRP2	Vesicular Transport Assay			Intestinal Barrier Renal Barrier Hepatic Barrier
Mrp2 - Rat Vesicular Transport Assay	Mrp2 - rat	Vesicular Transport Assay			Intestinal Barrier Renal Barrier Hepatic Barrier
MRP3 Vesicular Transport Assay	MRP3	Vesicular Transport Assay			Intestinal Barrier Hepatic Barrier
MRP4 Vesicular Transport Assay	MRP4	Vesicular Transport Assay			Blood-brain-barrier Renal Barrier Hepatic Barrier
MRP5 Vesicular Transport Assay	MRP5	Vesicular Transport Assay			Hepatic Barrier

Description

The vesicular transport assay is performed using inside out vesicles from membranes expressing the transporter of interest. The method can be applied to measure either inhibitory or substrate type interactions with ABC efflux transporters. The vesicular transport inhibition assay is a robust and quick assay suitable for screening large numbers of compounds. The read-out of the assay is easy to interpret and suitable for IC₅₀ determinations (ITC reference ). The success of substrate assays depends very much on the passive permeability of the compound. High permeability compounds will diffuse out of the vesicles and therefore will not be detected as substrates.

SOLVO Biotechnology offers vesicular transport substrates studies in three levels. The launch of the given Part(s) depend(s) on the success of the previous Part(s).

Part 1 – Vesicular Transport substrate feasibility studies: pilot experiments to determine the feasibility of testing

Part 2 – Vesicular Transport substrate characterization studies: Detailed characterization of the transport, determination of KM and Vmax

Part 3 – Optional Part for characterizing potential drug-drug interactions

Technology background