Vesicular Transport Assays
The vesicular transport assay is performed using inside out vesicles from membranes expressing the transporter of interest. The method can be applied to measure either inhibitory or substrate type interactions with ABC efflux transporters. The vesicular transport inhibition assay is a robust and quick assay suitable for screening large numbers of compounds. The read-out of the assay is easy to interpret and suitable for IC50 determinations (ITC reference ). The success of substrate assays depends very much on the passive permeability of the compound. High permeability compounds will diffuse out of the vesicles and therefore will not be detected as substrates.
SOLVO Biotechnology offers vesicular transport substrates studies in three levels. The launch of the given Part(s) depend(s) on the success of the previous Part(s).
Part 1 – Vesicular Transport substrate feasibility studies: pilot experiments to determine the feasibility of testing
Part 2 – Vesicular Transport substrate characterization studies: Detailed characterization of the transport, determination of KM and Vmax
Part 3 – Optional Part for characterizing potential drug-drug interactions