| Service | Cell Line | Transporter | Technology | Membrane Type | Probe Substrate | Read Out | Reference Inhibitor | Positive Control | Dynamic Range |
|---|---|---|---|---|---|---|---|---|---|
| Hepatocyte Uptake Assay | Hepatocyte Uptake Assay |
The SOLVO hepatocyte uptake assays are performed with single or multiple donor cryopreserved human hepatocytes (Celsis IVT, Neuss, Germany). All cryopreserved hepatocyte lots used in the experiments have been validated by SOLVO for transporter mediated uptake of prototypical substrates. In those validation experiments the prototypical probe substrates taurocholate (TC), estrone-3-sulfate (E3S) and 1-methyl-4-phenylpyridinium (MPP+) confirmed the NTCP, OATP and OCT-related active uptake capacity of the hepatocytes, respectively.
SOLVO offers two methods for studying uptake into hepatocytes: the oil-spin and the filterplate technology. Both methods provide a reliable approach to determine whether Test Articles (TAs) are actively (through a transporter) or passively (by diffusion) taken up into hepatocytes. Accumulation of the TAs in cryopreserved human hepatocytes is generally determined by incubating TA at two concentrations using two to four incubation time points, at 37°C and on ice. Intracellular accumulation of TAs at 37°C represents active uptake, while incubation on ice represents passive diffusion of the TAs.
Learn more about the Technology background of the Hepatocyte Uptake Assay
