Product | Cell Line | Transporter | Technology | Membrane Type | Probe Substrate | Readout | Reference Inhibitor | Positive Control | Dynamic Range |
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SB-TranSelect-Control | Uptake Transporter Assay |
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SB-TranSelect-MATE1 | MATE1 |
Uptake Transporter Assay |
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SB-TranSelect-MATE2-K | MATE2 |
Uptake Transporter Assay |
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SB-TranSelect-OAT1 | OAT1 |
Uptake Transporter Assay |
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SB-TranSelect-OAT3 | OAT3 |
Uptake Transporter Assay |
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SB-TranSelect-OATP1B1 | OATP1B1 |
Uptake Transporter Assay |
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SB-TranSelect-OATP1B3 | OATP1B3 |
Uptake Transporter Assay |
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SB-TranSelect-OCT1 | OCT1 |
Uptake Transporter Assay |
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SB-TranSelect-OCT2 | OCT2 |
Uptake Transporter Assay |
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SB-TranSelect-Supplement | Uptake Transporter Assay |
Packaged in a convenient thaw-and-use format, SOLVO TranSelect™ enables your lab to study solute carrier (SLC) uptake transporters with industry-leading performance engineered by SOLVO Biotechnology, the pioneer in drug transporter assay development. This new product line aims to complement our traditional PREDICELL™ Kits in the field of ready-to-use uptake transporter assays. The concept of TranSelect™ is simple: cryopreserved transporter-overexpressing and control cells are shipped from SOLVO to your lab at ultralow temperature – in liquid nitrogen vapor or on dry ice – via reliable couriers. Once thawed, you seed the cells into a plate format of choice, keep them in a humidified CO2 incubator overnight, and measure the accumulation of substrate or inhibition of transport 18-24 hours after seeding. To guarantee the high standards required for regulatory studies, cells undergo dual quality control at SOLVO: each batch is tested for viability and transporter activity prior to and after freezing.
Advantages
TranSelect™ Cryopreserved Cells offer exceptional flexibility and security in planning your experiments.
What is delivered to you:
* Dual Quality Control: each batch is tested for viability and transporter activity prior to and after freezing.
** The assay setup recommended for regulatory studies contains transporter-expressing and control cells seeded in triplicate wells for all conditions to assure a robust and fully controlled design. For more screening-like approaches you may want to order transporter-expressing cells only which can be used with inhibitors as control.
Assay performance
TranSelect™ technology minimizes intra-lot and inter-lot variation, thus ensuring constant assay performance and reproducibility of results. Here, the time-dependent accumulation of tenofovir (Figure 1) and inhibition of OAT1-mediated tenofovir uptake by probenecid (Figure 2) are compared across TranSelect™ OAT1 vials from the same lot as well as vials from different lots.
Figure 1 - TranSelect™ OAT1: TranSelect OAT1 cells exhibit low inter- and intra-lot variability in time-dependent specific accumulation of tenofovir.
Figure 2 - TranSelect™ OAT1: Inhibition of tenofovir transport using TranSelect™ OAT1 cells. Minimal inter- and intra-lot variability was observed, enabling consistent IC50 determinations.
*Competitor OATP1B1 cells require treatment with butyrate that necessitates replacement of growth medium 3-4 hours post seeding. Some cells that have not yet firmly attached may be lost at this step, leading to lower signal. TranSelect™ cells do not require this extra step as the Supplement can be added to the growth medium before seeding.
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