PreadyPort™

Product Cell Line Transporter Technology Membrane Type Probe Substrate Read Out Reference Inhibitor Positive Control Dynamic Range
SB PP BCRP 24-well MDCKII-BCRP BCRP
Monolayer Assay
SB PP BCRP 96-well MDCKII-BCRP BCRP
Monolayer Assay
SB PP MDR1 24-well MDCKII-MDR1 MDR1/P-gp
Monolayer Assay
SB PP MDR1 96-well MDCKII-MDR1 MDR1/P-gp
Monolayer Assay
SB PP CTRL 24-well MDCKII Monolayer Assay
SB PP CTRL 96-well MDCKII Monolayer Assay
SB PP BCRP mix 24TW MDCKII-BCRP/MDCKII (1:1) BCRP
Monolayer Assay
SB PP MDR1 mix 24TW MDCKII-BCRP/MDCKII (1:1) MDR1/P-gp
Monolayer Assay
SB PP MDR1 mix(1:1) 96MW MDCKII-MDR1/MDCKII (1:1) MDR1/P-gp
Monolayer Assay
SB PP MDR1 mix(3:1) 96MW MDCKII-MDR1/MDCKII (3:1) MDR1/P-gp
Monolayer Assay
SB PP MDR1 mix(c) 96MW MDCKII-MDR1/MDCKII (custom) MDR1/P-gp
Monolayer Assay
SB PP BCRP-OATP2B1 24TW MDCKII-OATP2B1 and -BCPR BCRP
Monolayer Assay
SB PP BCRP-OATP2B1 96MW MDCKII-OATP2B1 and -BCPR BCRP
Monolayer Assay
SB PP BCRP mix(1:1) 96MW MDCKII-BCRP/MDCKII (1:1) BCRP
SB PP BCRP mix(3:1) 96MW MDCKII-BCRP/MDCKII (3:1) BCRP
SB PP OATP2B1-BCRP mix 24TW MDCKII-OATP2B1 and -BCRP/MDCKII (1:1) OATP2B1
BCRP
SB PP OATP2B1-BCRP mix 96TW MDCKII-OATP2B1 and -BCRP/MDCKII (custom) OATP2B1
BCRP

Description

PreadyPortTM, the Ready-to-use Transfected MDCKII Monolayer Assay Kits, contain differentiated MDCKII cells expressing a selected drug transporter on 24- or 96-well cell culture insert plates.

PreadyPortTM Kits are suitable for both substrate and inhibition assessments and are often used to model the net transport events of important fluid compartment barriers in the organism that expresses a specific transporter at a high level.

The PreadyPortTM Kits allow researchers to perform transporter interaction studies on monolayers without bothering about cell line licensing and culturing. The innovative shipping medium, developed and patented by Advancell, preserves the properties of the barrier throughout transportation, allowing us to provide customers with a robust, ready-to-use drug transport assay kit.
Monolayer assays using transfected MDCKII cells are widely recognized tools, recommended by the FDA, to study the interaction of drugs with efflux transporters.

SOLVO Biotechnology introduced PreadyPortTM in collaboration with ADVANCELL and ReadyCell (Spain).

Applications

  • Substrate assessment (direct transport studies) on MDR1, BCRP, OATP2B1
  • Inhibitor assessment (drug-drug interaction studies) on MDR1, BCRP, OATP2B1
  • Models the net transport events of barriers where MDR1, BCRP, OATP2B1 are present.
 
Features
  • Differentiated MDCKII-MDR1 or MDCKII-BCRP or MDCKII-BCRP-OATP2B1 monolayer (11 day system)
  • Ready-to-use
  • The Kit can be used up to 7 days after reception
  • 24-TranswellTM (Costar) or 96-Multiwell (Millipore) format
  • Up to 4 days of transportation and storage at room temperature in proprietary shipping medium
  • Shipping medium is easy to remove after liquefaction at 37 °C
  • Available under a Limited Single-use License without extra charge
  • Sample Assay Protocol
  • Sample Plate Layouts

Benefits

  • Available on demand
  • User-friendly system and easy-handling
  • Flexibility: a window of 4 days for transport measurements
  • Adaptable to automation
  • Transporter experiments without in-house cell propagation
  • Transporter experiments without in-house cell line development or acquisition





Learn more about the PreadyPortTM Kit and validation data.

We highly recommend reading the “Chemicals and instruments needed” and the “Tips for Success guide” before your PreadyPortTM Kits arrive. Please request them from your local account manager

 

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