PreadyPort™
Description
PreadyPort
TM, the Ready-to-use Transfected MDCKII Monolayer Assay Kits, contain differentiated MDCKII cells expressing a selected drug transporter on 24- or 96-well cell culture insert plates.
PreadyPort
TM Kits are suitable for both substrate and inhibition assessments and are often used to model the net transport events of important fluid compartment barriers in the organism that expresses a specific transporter at a high level.
The PreadyPort
TM Kits allow researchers to perform transporter interaction studies on monolayers without bothering about cell line licensing and culturing. The innovative shipping medium, developed and patented by ReadyCell, preserves the properties of the barrier throughout transportation, allowing us to provide customers with a robust, ready-to-use drug transport assay kit.
Monolayer assays using transfected MDCKII cells are widely recognized tools, recommended by the FDA, to study the interaction of drugs with efflux transporters.
SOLVO Biotechnology introduced PreadyPort
TM in collaboration with ReadyCell (Spain).
Applications
- Substrate assessment (direct transport studies) on MDR1, BCRP, OATP2B1
- Inhibitor assessment (drug-drug interaction studies) on MDR1, BCRP, OATP2B1
- Models the net transport events of barriers where MDR1, BCRP, OATP2B1 are present.
Features- Differentiated MDCKII-MDR1 or MDCKII-BCRP or MDCKII-BCRP-OATP2B1 monolayer (11 day system)
- Ready-to-use
- The Kit can be used up to 7 days after reception
- 24-TranswellTM (Costar) or 96-Multiwell (Millipore) format
- Up to 4 days of transportation and storage at room temperature in proprietary shipping medium
- Shipping medium is easy to remove after liquefaction at 37 °C
- Available under a Limited Single-use License without extra charge
- Sample Assay Protocol
- Sample Plate Layouts
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Benefits
- Available on demand
- User-friendly system and easy-handling
- Flexibility: a window of 4 days for transport measurements
- Adaptable to automation
- Transporter experiments without in-house cell propagation
- Transporter experiments without in-house cell line development or acquisition
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Please request details from your local account manager.