ATPase Assays
| Service | Cell Line | Transporter | Technology | Membrane Type | Probe Substrate | Read Out | Reference Inhibitor | Positive Control | Dynamic Range |
|---|---|---|---|---|---|---|---|---|---|
| BCRP ATPase Assay | BCRP |
ATPase Assay |
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| Bsep - Mouse ATPase Assay | Bsep - mouse |
ATPase Assay |
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| MDR1/P-gp ATPase Assay | MDR1/P-gp |
ATPase Assay |
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| Mdr1b-rat ATPase Assay | Mdr1b - rat |
ATPase Assay |
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| MRP2 ATPase Assay | MRP2 |
ATPase Assay |
Description
The ATPase assay is an ideal, cost effective assay to identify interaction between test compounds and ABC (efflux) transporters. The assay is performed on isolated cell membranes expressing the transporter of interest. The ATPase assay is unique in that it can distinguish between an inhibitor and a substrate in one assay (one plate). Moreover, the assay works independently from the passive permeability of the test compound, in contrast to cellular assays like MDCKII monolayers. Due to its low costs and simple design the ATPase assay is suitable for screening large numbers of compounds in a relatively short time.
Learn more about the Technology background of the ATPase Assay
