ATPase Assays

Service Cell Line Transporter Technology Membrane Type Probe Substrate Read Out Reference Inhibitor Positive Control Dynamic Range
BCRP ATPase Assay BCRP
ATPase Assay
Bsep - Mouse ATPase Assay Bsep - mouse
ATPase Assay
MDR1/P-gp ATPase Assay MDR1/P-gp
ATPase Assay
MRP2 ATPase Assay MRP2
ATPase Assay
MRP3 ATPase Assay MRP3
ATPase Assay
Mdr1b-rat ATPase Assay Mdr1b - rat
ATPase Assay

Description

The ATPase assay is an ideal, cost effective assay to identify interaction between test compounds and ABC (efflux) transporters. The assay is performed on isolated cell membranes expressing the transporter of interest. The ATPase assay is unique in that it can distinguish between an inhibitor and a substrate in one assay (one plate). Moreover, the assay works independently from the passive permeability of the test compound, in contrast to cellular assays like MDCKII monolayers. Due to its low costs and simple design the ATPase assay is suitable for screening large numbers of compounds in a relatively short time.

Learn more about the Technology background of the ATPase Assay